One shot mode | Continuous mode | ||
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Focusing position | Dry objective | Interface between air and cover glass | |
Immersion objective | Interface between sample (cultured liquid) and cover glass |
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Offset method | controlled by software | Compensation for shift of observation position toward the focusing plane is by Z-axis control | |
Observation methods | Brightfield, DIC and fluorescence | Brightfield and fluorescence | |
FN limitation | Light volume is low at the image perimeter for F.N. 22 when using 2x, 4x, 10x objectives | ||
Focusing speed | Depending on software implementation | Sampling rate 10ms | |
Focusing accuracy | Dry objective | Standard deviation: σ =0,1µm | |
Immersion objective | Standard deviation: σ =0,07µm | ||
Laser | Laser diode 785nm, 5µW |